Quality and Protein Characterization of Triticale Lines with the Pair of HMW Subunits 5+10 and 2+12

摘要

Genetic lines that differ at one or more loci coding for proteins can be successfully used to elucidate the relationships between protein composition and functionality. A set of fifteen near-isogenic triticale lines has been characterized by protein composition (SDS-PAGE, SE-HPLC) and quality measurements (Mixograph). This allowed the effects on quality of different loci coding for gluten proteins to be evaluated using a common genetic background. These new triticale lines were developed using the triticale Rhino, possessing 1RL.1DL or 1AL.1DL translocations and contain genes corresponding to either the pair 5+10 or 2+12. The triticale lines Rigel and Trim were used in the translocation. The main variables that were found to affect quality were thenumber and type of High Molecular Weight Glutenin Subunits (HMW-GS), the relative amounts of glutenins and gliadins and the presence of chain terminator proteins. The mixograph studies support the SE-HPLC data in which, higher UPP correlates with higherpeak development time.