Entamoeba Histolytica Strain with a Non-described Chitinase Genotype in Mexican Patients with Amoebic Liver Abscess

摘要

We described an E. histolytica genotype detected in the drainage material of amoebic liver abscess from six Mexican patients. Five of theme experiences an ALA that needed drainage due to the risk of rupture and one developed a lung empyema consequence of the opening of the primary liver abscess to thoracic cavity. The material obtained through a sonography guide hepatic puncture was treated for DNA extraction. The DNA was used to amplify the polymorphic region of chitinase gene. The PCR products were sequenced, translated, aligned and compared with the already chitinase sequences reported in Gen Bank data base. The alignment of our sequence did not show identity with reported data so it was registered in the Gen Bank data base with the reference number EF 445962. Our results support the previous observations of the possible particular geographic distribution of E. histolytica and E. dispar strains all over the world and their high genetic variability.