Encapsulation of Enzymes inside Peptide Nanotube for Hydrogen Peroxide Detection

摘要

Biosensor matrixes have been developed using hydrogen peroxidase (HRP) as a model enzyme and peptide nanotube (PNT). The enzymes are encapsulated inside self-assembled PNT. The catalytic activity and the stability of the encapsulated HRP have been investigated. The electrochemical property of the PNT was characterized using cyclic voltammetry (CV) and compared with free enzymes. The formation of PNT was conducted by NH_2-Phe-Phe-COOH dipeptides as building blocks. Scanning electron microscope (SEM) and atomic force microscope (AFM) were used to confirm the hollow nanostructure of the PNT. The encapsulation of HRP inside PNT was proved using scanning transmission electron microscope (STEM) and UV-Vis spectrophotometer. The result showed that the enzyme sensing matrix system incorporated with PNT provided an attractive advantage for a new type of electrochemical biosensors.