A versatile strategy for electrochemical detection of hydrogen peroxide as well as related enzymes and substrates based on selective hydrogen peroxide-mediated boronate deprotection

摘要

We reported a versatile strategy for electrochemical determination of H_2O_2 as well as related enzymes and substrates by combining the advantages of H_2O_2-mediated selective boronate deprotection and paminophenol for the first time. H_2O_2 is selectively detected through the determination of p-aminophenol generated from H_2O_2-mediated boronate deprotection of p-aminophenylboronic acid. This method allows H_2O_2 detection in the range from 1 μM to 1 mM with a detection limit of 0.8 μM. Since many enzymes and their substrates can be detected through the determination of H_2O_2, we extend this method to detect related enzymes and their substrates using glucose and glucose oxidase as representative analytes. There is a good linear relationship between the current responses and GOx activity from 0.01 U/mL to 0.8 U/mL with a detection limit of 0.01 U/mL. The method is promising for broad and important applications, such as driver alcohol detection as well as clinical analysis of glucose, uric acid, lactate, glutamate, and so on.