Glucose Oxidase/Polypyrrole Biosensor Fabricated by Galvanostatic Electropolymerization Accompanied with a Potential Jumping

摘要

A galvanostatic glucose oxidase (GOD{sub}x)/polypyrrole (Ppy) biosensor fabricated through a potential jumping process was reported. In spite of the instantaneous voltage jumping from 0.95 V to 1.7 V versus Ag/AgCl in the galvanostatic polymerization of pyrrole with commercial GOD{sub}x, this GOD{sub}x enzyme still could be successfully bundled with polypyrrole (Ppy). And, the results also confirmed that this glucose sensor not only possessed of better sensitivity to glucose than that fabricated from potentiometry or mild voltage elevating galvanometry for the similar preparing conditions but also exhibited two-week long-term stability. Finally, the kinetic data of Michalis constant (K{sub}m) and Maximum current (I{sub}m) for the optimized sensor were derived. The discovery this electro-modification process may disclose a novel method for fabricating the other enzyme biosensors.