Pulsed EPR Distance Measurements on Spin-Labeled Proteins and Protein Complexes: Opportunities and Limitations

摘要

Pulsed EPR measurements such as pulsed ELDOR or double-quantum EPR allow for the measurement of spin-to-spin distances on length scales between 1.5 and 6 nm that are comparable to the extension of a protein. With appropriate measurement protocols and data analysis techniques distance distributions can be obtained, so that even flexible domains of proteins can be characterized.