Layered thin films composed of concanavalin A (Con A) and sugar-bearing polymers were prepared by a layer-by-layer deposition of Con A and the polymer on a solid surface and the sugar-induced disintegration was studied. The Con A/polymer layered film was disintegrated upon addition of sugars in the solution owing to the preferential binding of the sugars to the binding site of Con A in the film. The disintegration rate was dependent on the type of sugar and its concentration. Thin films composed of avidin and iminobiotin-Iabelled polyethyleneimine (ib-PEI) can be disintegrated in response to pH changes because the affinity of ib-PEI is lowered by protonation. The avidin-ib-PEI film is sensitive to biotin and analogues. Thus, the avidin thin films can be disintegrated by H+ ion or biotin as external stimuli.
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机译：通过层A的层A的层沉积和固体表面上的聚合物和糖诱导的崩解制备的层状薄膜由Con A的层和含糖聚合物组成。由于糖的优先结合在薄膜中的Con A的结合位点，在溶液中加入糖时，将Con A /聚合物层状膜崩解。崩解率取决于糖的类型及其浓度。薄膜响应于pH变化，可以崩解由抗生物素蛋白和Iminobiotin-Ibelled聚乙烯亚胺（IB-PEI），因为通过质子化降低IB-PEI的亲和力。抗生物素IB-PEI膜对生物素和类似物敏感。因此，抗生物素蛋白薄膜可以通过H +离子或生物素作为外部刺激崩解。
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