Expression of a Bombyx mori Tyramine Receptor in HEK-293 Cells and Action of a Formamidine Insecticide

摘要

B96Bom was cloned from the silkworm Bombyx mori and used for stable expression in HEK-293 cells. The expressed B96Bom receptor led to an attenuation of forskolin-stimulated intracellular cAMP production in response to tyramine. The attenuation was abolished by yohimbine. Octopamine was less effective than tyramine. Tyramine inhibited [3H]tyramine binding to the B96Bom receptor more potently than octopamine and dopamine. The acaricide/insecticide chlordimeform and its metabolite demethylchlordimeform inhibited [3H]tyramine binding but did not show significant agonist activity. The stable expression system might prove useful for the discovery of novel ligands and bioactive .compounds.