MICRO REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION SYSTEM FOR CLINICAL DIAGNOSIS

摘要

This paper reports a micromachined Reverse-Transcription Polymerase Chain Reaction (RT-PCR) system integrated with a polydimethylsiloxane (PDMS)-based pneumatic pumping/valving system for fast cloning and clinical diagnosis of RNA-based molecules such as RNA viruses or mRNA. Successful amplification of complementary DNA (cDNA) synthesis from the 10723-base dengue-2 virus templates was carried out by using on-chip microfluidic and temperature control systems in an automatic format. The integrated 2-step micro-RT-PCR system could be a crucial tool for infectious disease detection.