Diagnosis of tospoviruses by reverse-transcription polymerase chain reaction

摘要

Immunocaptured virus or RNA extracted from infected tissue, and primers corresponding to segments of the L genomic RNA, were used to identify tomato spot wilt tospovirus (TSWV) by reverse-transcription-PCR. Primers corresponding to sections of the Sgenomic RNA species were used to detect impatiens necrotic spot tospovirus (INSV). A primer mixture containing sequences for amplified segments of both the L and S viral genomic RNAs was used to detect and differentiate both TSWV and INSV in the same multiplex reaction mixture by producing a PCR product of characteristic size. A second set of universal primers containing sequences for amplification of a different section of the S RNA was also used to detect all tospoviruses tested.