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An Integrated Software Solution for Real-time PCR Analysis Based on Microfluidic Biochip

机译:基于微流体Biochip的实时PCR分析综合软件解决方案

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摘要

In this paper, we present an integrated and automated prototype system which has been developed for real-time polymerase chain reaction (PCR) analysis based on microfluidic PCR array chips. The system integrates the PCR thermal cycling and optical detection capabilities to enable real-time fluorescence imaging and image processing for data analysis. The main advantage of the system is that it provides a solution that can rapidly perform and evaluate PCR experiment simultaneously on microfluidic PCR array chips. The system has demonstrated fast and efficient on-chip real-time PCR analysis using human genomic DNA samples. The implementation of the system integration is a multi-thread Windows software with component structure which is written in Visual C++.
机译:在本文中,我们提出了一种集成和自动化的原型系统,该系统已经开发用于基于微流体PCR阵列芯片的实时聚合酶链反应(PCR)分析。该系统集成了PCR热循环和光学检测能力,以实现数据分析的实时荧光成像和图像处理。该系统的主要优点是它提供了可以在微流体PCR阵列芯片上同时快速执行和评估PCR实验的解决方案。该系统使用人类基因组DNA样品证明了快速和有效的片上实时PCR分析。系统集成的实现是一个多线程Windows软件,其中包含以Visual C ++编写的组件结构。

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