DESIGN AND FINITE ELEMENT OF PDMS-BASED MICROFLUIDICS PCR DEVICES WITH GLASS VAPOR BARRIER STRUCTURE

摘要

In this paper, we applied CoventorWare~(TM), commercial finite element MEMS simulation software, for design and simulation of a microfluidics control and vapor diffusion for polydimethylsiloxane (PDMS) devices to control and reduce vaporization at elevated temperatures and improve the reliability of polymerase chain reation (PCR). We have redesigned the structure between the PDMS and the implant by depositing an extra stack of glass, a vapor barrier, at the top of the device. Because of this improved method, we can illuminate the potential and practical applications of PCR due to reducing the loss of microfluidic samples in the amplification stage.