Dimerization of penicillin V as deduced by frontal derivative chromatography

摘要

The aggregation pattern of penicillin V (PCV) is investigated by frontal derivative chromatography on Sephadex G-10 columns. The height and position of the trailing peak, computer-simulated on the basis of the stepwise aggregation model, are in excellent agreement with the observed data, whereas those simulated on the basis of the trimer - dodecamer model remarkably differ from the observed data, particularly in dilute solutions. Thus, PCV forms dimers and self-associates stepwise.