IMMUNOASSAY AND SEQUENCE-SPECIFIC DNA DETECTION ON A MICROCHIP USING ENZYME-AMPLIFIED ELECTROCHEMICAL DETECTION

摘要

A CMOS fabricated silicon microchip was used as a platform for immunoassays and DNA synthesis and hybridization. The active silicon chip has over 1,000 active electrodes that can be individually addressed for both synthesis of DNA and protein attachment to a membrane on the chip surface. The active chip can be used for the detection of various analytes at the chip surface via the attachment of enzymes on captured oligonucleotides or antibodies.