The nano Doughnut-like Copper periodic structure is fabricated by an electro-plating method on patterned Ni template. The diameter of the cover Cu doughnut hole can be controlled by a plating duration. This structure can be applied in a biosensor due to surface plasmonic resonance, and for novel optical device with variable hole diameter and period of that array. The Magnetic Force Microscope (MFM) was used for evaluating the bio-sensing response under the addition of magnetic proteins (metallothionein-MT, Mn2Cd5 Metallothionein-MMT, and Ferritin) on that sample before and after magnetization. In addition, the optical properties before and after magnetization were measured and scanned by Fourier transform infrared spectroscopy (FTIR). It is noted that some interesting behavior were observed in the spectra and transmitting ratio under different polarization incident conditions.
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