Application of neutrophils chemiluminescence test in the differential diagnosis of bronchial asthma and recurrent respiratory tract infection in the remission period in children

摘要

In this study we present the comparison of neutrophils activity, measured in whole blood by a chemiluminescence test in children with bronchial asthma and recurrent respiratory tract infections (RRTI). We estimated five parameters of chemiluminescence: I - a time of spontaneous stabilization of CL (following counts remain on the same level with a tendency to decrease), II - a time of maximal CL excitation (time after addition of stimulator during which the cells emit the highest amounts of photons), III - an average value of spontaneous CL (from the beginning of measurement to the addition of f-MLP), IV - a maximal value of CL after stimulation and V - an area under CL curve. The values of parameters III, IV and V were significantly lower in the children with rrti (p < 0.0001) than those in the control group. The neutrophils' activity, reflected in measured parameters of CL, was several times greater in the children with bronchial asthma than that in the control (p < 0.0001) and RRTI (p < 0.0001) groups. A deterioration of oxygen metabolism of neutrophils, measured by CL test, was demonstrated in children with rrti.