Electrochemical interactions in films combining conductive polymers with enzymes or redox proteins may provide a basis for highly sensitive biosensors.For example,vectorial electron transport from electrode to enzyme to substrate might be mediated by a conductive polymer faster than in its absence,greatly improved signal to noise ratios for catalytic currents.Various transducer configurations employing such films for sensitive analytical measurements of resistance or impedance might also be envisioned.1 In this paper we explore films grown on graphite and gold electrodes with inner layers of conductive ionic polymer sulfonated polyaniline(SPANI)and outer layers of proteins myoglobin or horseradish peroxidase.Electrochemical polymerization of the inner layer of SPANI vs.the adsorption of pre-formed SPANI onto a layer of cationic polyion were compared.This paper discusses the construction and characterization of these films.
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