Coat Protein Sequence of an Egyptian BYDV-PAV Isolate

摘要

BYDV-PAV is the most common strain in Egypt. Immunocapture IC/RT-PCR procedure was used to amplify fragments specific for BYDV-PAV. The system was further validated by sequencing fragments amplified. The cDNA of CP was inserted into plasmid to construct expression plasmid. The recombinant plasmid was identified by using restriction enzymes and sequenced to confirm PAV-CP gene in plasmid. The sequence of the coat protein of the PAV strain was identified and its amino acid sequence was deduced. Sequence comparison of the CP coding region between the PAV strains of BYDV liave been done, and revealed the highest identity with the Moroccan strain (99%).