Identification and characterization of Rhizoctonia solani using PCR/RFLP and a pine bioassay

摘要

Ninety-nine Rhizoctonia solani isolates from various origins were first classified within groups and sub groups according to the concept of anastomosis group. We then developed the specific primers RSI and RS4 based on the ITS sequences of the nuclear rDNA. The PCR products were digested with seven restriction enzymes: Ase I, Ava II, Bse GI, Hinc II, Mse I, Bspl 1, and Cfr 13 1. The results showed that there is a perfect correlation between anastomosis classification and molecular systematic. The PCR/RFLP method allowed us to detect and identify R,solani in naturally infected pines seedling at a very early stage of attack. The pathogenicity of the strains belonging to the main anastomosis groups occurring in France was easily determined using a pine bioassay.