ACID CITRUS FRUIT CULTIVAR IMPROVEMENT VIA INTERPLOID HYBRIDIZATION

摘要

We have conducted several Interplold crosses between fertile somatic hybrids such as 'Hamlin' + 'Femminello', 'Key' lime + Valencia', 'Valencia' + 'Femminello', 'Milam' + 'Femminello' and 4X 'Femminello' and various diploid acid-fruit cultivars to generate seedless triploids with horticultural-|y acceptable fruit and improved cold-hardiness (inherited from sweet orange), suitable for production in sub-tropical regions. Embryo rescue was utilized to recover triploid progeny about three months after pollination. Embryos in different developmental stages were grown on Murashige and Tucker medium supplemented with 500 mg/L malt extract. A total of fifty three triploids and twenty two tetraploids were recovered from different cross combinations.