Data analysis in green fluorescent protein based fluorescence resonance energy transfer

摘要

Green fluorescent protein based fluorescence resonance energy transfer is widely used i nstudies of inter- and intra-molecular dynamic interactions in alive cells. But it is a trade off between quantitative measurement and real time measurement because of the emission cross talk. In steady of using more than one excitation wavelength and several sets of filters, we proposed a more efficient correction scheme which need only a singel excitation and one filter set. This method makes use of the known emission spectra of the fluorophores and can be run in real time. The validity and advantages were demonstrated by experiments with cameleons.