ANALYSIS OF MODIFICATION AND DEMODIFICATION OF PROTEINS WITH UBIQUITIN-RELATED PROTEINS BY MEANS OF FRET (FLUORESCENCE RESONANCE ENERGY TRANSFER)

摘要

The invention relates to a method for analysing modifications of proteins with ubiquitin-related proteins and demodification of proteins which are concatenated with ubiquitin-related proteins. The invention also relates to a method for determining substances which influence said modifications or demodifications, in addition to a method for detecting a defect in said modifications or demodifications. Furthermore, the invention relates to a method for identifying target proteins for modification with ubiquitin-related proteins and a method for producing a pharmaceutical composition. The invention further relates to a kit, a fusion protein, a cell and an isopeptidase substrate and the use thereof in the analysis and/or diagnosis of modification or demodification of proteins with ubiquitin-related proteins.