GLUCOSE QUANTIFICATION IN COMMERCIAL SERUMS BY MEANS OF AMPEROMETRIC BIENZIMATIC BIOSENSOR BASED ON A BIOCOMPOSITE RIGID MATRIX.

摘要

In the last years the study of amperometrics bienzimatics biosensors has grown,due to the possibility of increasing the sensitivity when more than one enzyme is used [1-5].In these investigations the biological material is immobilized superficially.The main with this kind biosensors is their useful time,which,generally is not more than few days.The objective of the present work consists on the construction and optimization of an amperometric bienzimatic biosensor of type for the quantification of glucose in serums.The biosensor is based on the combined immobilization of the enzymes glucose oxidase(GOD)and horseradish peroxidase(HRP)in a biocomposite of rigid matrix.When this immobilization technique is used,the advantages in terms of biosensor lifetime and the possibility to reduce the protoheme group(HRP)to the active form when exist an intimate contact of the enzymes with the graphite powder,applying a potential of-300 mV vs Ag/AgCl the active place is regenerated [6].