基于自组装原位生长法制备聚苯胺/CNTs纳米复合物修饰叉指电极的葡萄糖生物传感器

摘要

采用自组装原位生长制备基于聚苯胺/CNTs纳米复合物修饰叉指电极的葡萄糖生物传感器.首先采用自组装的方式制备CNTs修饰叉指电极，并采用对甲苯磺酸掺杂聚苯胺，原位在修饰电极表面生长聚苯胺，制备了PANI/CNTs修饰电极；然后将葡萄糖氧化酶固定在修饰电极表面制备了葡萄糖生物传感器.采用拉曼光谱、扫描电子显微镜、原子力显微镜等对所制备的PANI/CNTs复合物进行了表征分析；采用循环伏安法和电化学阻抗法研究了修饰电极的电化学行为.传感器性能评价结果表明：在最优化条件下，葡萄糖浓度与响应电流在0.5~30 mmol/L范围内呈现良好的线性关系，响应灵敏度为62.17μA/（mmol·L-1），线性相关系数为0.997，检出限为0.15 mmol/L（S/N=3），并具有良好的重现性和稳定性.%Glucose biosensors based on self assembled-in situ polymerized polyaniline /CNTs nanocomposite modified interdigitalelectrode were prepared. Firstly, CNTs modified interdigital electrode was prepared by self assembling process, and PANI/CNTs was prepared using p-toluene sulfonic acid doped polyaniline in situ polymerized aniline on the electrode surface;then glucose biosensor was prepared by immobilization of glucose oxidase on the as prepared modified electrode surface. The PANI/CNTs nanocomposites were characterized by Raman spectra analysis, scanning electron microscopy and atomic force microscopy;electrochemical behaviors of the modified electrode were characterized by cyclic voltammetry and electrochemical impedance method. The results showed that under the optimum conditions, glucose concentration and the response current showed a good linear relationship in the range of 0.5-30 mmol/L with the response sensitivity of 62.17 μA/(mmol·L-1), the linear correlation coefficient of 0.997, and the detection limit of 0.15 mmol/L (S/N=3). The biosensor also showed good stability and reproducibility.