Class-specific regulation of immunoglobulin production by rose bengal in human B cell lines and peripheral blood lymphocytes

摘要

We have previously reported that Rose Bengal (RB) can enhance IgE prduction in rat spleen lymphocytes without increasing the production of IgG and IgM. To examine the effect of RB in human B cell lines, U-266 (IgE producer), HMy-2 (IgG producer) and NAT-30 (IgM producer) cells were cultured with RB, and the medium immunoglobulin (Ig) level was measured by ELISA. Though RB enhanced IgE production in rat lymphocytes, it inhibited IgE production in U-266 and IgG production in HMy-2 cells in dose dependent manner. In NAT-30 cells, RB slightly inhibited IgM production at 0.1 #mu#M, while it enhanced production at 10 #mu#M. These results suggest that RB directly modulates Ig production in B cells, and that the modulatory effect is different between rat lymphocytes and human cell lines. Therefore, we examined the effect of RB on Ig production in human peripheral blood lymphocytes. We found that RB did not enhance IgE production in lymphocytes as well as human cell lines.