Effects of serum and other medium components on suspension culture of CHO cells producing tissue plasminogen activator

摘要

Effects of the concentrations of serum and other medium components on cell metabolism in a suspension culture, in a spinner bottle, of CHO 1-15500 cells (ATCC CRL-9606) producing tissue plasminogen activator (tPA) were investigated in order to formulate an appropriate medium composition for perfusion culture. Ham's F-12 medium supplemented with 500 nM of methotrexate and 0-10% newborn-calf serum was employed. Cell and tPA concentrations were assayed using trypan blue and ELISA, respectively. Concentrations of glucose, lactate, glutamine and glutamic acid were determined enzymatically, and those of ammonia using a colorimetric method.