FCLA assisted Photo-Dynamic Diagnosis

摘要

Conventional Photo-Dynamic Diagnosis (PDD) has several serious limitations, such as autofluorescence disturbance, poor penetration ability of the exciting laser etc. All these problems are because the exciting laser wavelength at the main absorption peak of the photosensitizer (e. g. HpD) is too short. But to our knowledge, long wavelength He-Ne laser can sensitize appropriate sensitizer to produce singlet oxygen (~1O_2) without autofluorescence of the normal tissues. The ~1O_2 can react with FCLA (5-Fluoresceinyl Cypridina Luminescent Analog) to emit chemiluminescence at the peak wavelength 532nm, thus we can use a high sensitivity detector to image the emission from FCLA to make a localization of the cancer. This is a novel idea proposed by our group, and its feasibility was proved by our experiments.