Liquid chromatography combined with visible diode-laser-induced fluorescence detection for photosensitizers

摘要

A combination of conventional-size liquid chromatography and visible diode-laser induced fluorescence (DIO-LIF) detection has been developed and applied for the detection of a model photosensitizer, AlPcS$-2$/, in biological samples. Conventional fluorimetry was found to give unsatisfactory information. A detection limit of 2.1 $MUL 10$+$MIN@12$/ M (S/N $EQ 3) was obtained for AlPcS$-2$/ in both rat urine and faeces extracts using the DIO-LIF system, 20-fold lower than obtained with a conventional fluorescence detection system, when used in combination with chromatography.