首页> 外文会议>Conference on Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing >Observing dynamics of transparent samples by harmonically matched grating-based full-field quadrature phase interferometer
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Observing dynamics of transparent samples by harmonically matched grating-based full-field quadrature phase interferometer

机译:谐波基于光栅的全场正交相干扰仪观察透明样本的动态

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Our group has reported the use of harmonically matched diffraction grating for full-field quantitative phase imaging. In this paper, we show the improvement of this technique and the application in observing dynamics of transparent samples. By using the grating as a beam splitter/combiner in an interferometer, we are able to obtain non-trivial phase difference between the output ports of the grating. We have built a Mach-Zehnder interferometer using the holographic grating with 600 and 1200 lines/mm spacing. Two CCD cameras at the output ports of the grating-based Mach-Zehnder interferometer are used to record the full-field quadrature interferograms, which are subsequently processed to reconstruct the phase image. Since the two quadrature interferograms are acquired at the same time, the imaging speed of the system is limited only by the frame rate of the CCD cameras. We have demonstrated the capability of our system by observing dynamics of transparent samples.
机译:我们的小组报告使用谐波衍射光栅用于全场定量相显像。在本文中,我们展示了这种技术的改进以及观察透明样品动态的应用。通过在干涉仪中使用光栅作为分束器/组合器,我们能够在光栅的输出端口之间获得非平凡的相位差。我们已经使用600和1200线/ mm间距的全息光栅建立了Mach-Zehnder干涉仪。基于光栅的Mach-Zehnder干涉仪的输出端口的两个CCD相机用于记录全场正交干扰图,随后处理以重建相位图像。由于同时获得了两个正交干涉图,因此系统的成像速度仅受CCD摄像机的帧速率受到限制。我们通过观察透明样品的动态来展示了我们的系统的能力。

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