首页> 外文会议>Conference on Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing; 20080121,23-24; San Jose,CA(US) >Observing dynamics of transparent samples by harmonically matched grating-based full-field quadrature phase interferometer
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Observing dynamics of transparent samples by harmonically matched grating-based full-field quadrature phase interferometer

机译:基于谐波匹配的基于光栅的全场正交相位干涉仪观察透明样品的动力学

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Our group has reported the use of harmonically matched diffraction grating for full-field quantitative phase imaging. In this paper, we show the improvement of this technique and the application in observing dynamics of transparent samples. By using the grating as a beam splitter/combiner in an interferometer, we are able to obtain non-trivial phase difference between the output ports of the grating. We have built a Mach-Zehnder interferometer using the holographic grating with 600 and 1200 lines/mm spacing. Two CCD cameras at the output ports of the grating-based Mach-Zehnder interferometer are used to record the full-field quadrature interferograms, which are subsequently processed to reconstruct the phase image. Since the two quadrature interferograms are acquired at the same time, the imaging speed of the system is limited only by the frame rate of the CCD cameras. We have demonstrated the capability of our system by observing dynamics of transparent samples.
机译:我们的小组报告了谐波匹配衍射光栅在全场定量相位成像中的使用。在本文中,我们展示了该技术的改进以及在观察透明样品动力学方面的应用。通过将光栅用作干涉仪中的分束器/组合器,我们能够获得光栅输出端口之间的重要相位差。我们使用全息光栅以600和1200线/毫米的间距构建了马赫曾德尔干涉仪。基于光栅的马赫曾德尔干涉仪的输出端口上的两个CCD摄像机用于记录全场正交干涉图,随后对其进行处理以重建相位图像。由于同时获取两个正交干涉图,因此系统的成像速度仅受CCD摄像机的帧频限制。我们已经通过观察透明样品的动力学证明了我们系统的能力。

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