Deep UV Microcopy of prostate cancer tissue

摘要

Correctly diagnosing and staging prostate cancer continues to be a significant clinical challenge. Currently, the standardof care consists of a pathologist’s visual assessment of hematoxylin-and-eosin-stained (H&E) histological sections, anddesignation of a Gleason score based on the top two most common patterns. However, this process is subjective and thusprone to error. Further, lack of standard protocols for staining, makes quantitative analysis of stained tissues difficult.Therefore, there is a significant need to develop new quantitative methods that can provide robust, objective, andaccurate information of the aggressiveness and stage of prostate cancer.In this work, we seek to address this challenge using multi-spectral deep-UV microscopy of unstained tissue sections.This method yields valuable insight into the aggressiveness and stage of the disease due to its subcellular spatialresolution and high sensitivity to many endogenous biomolecules, including nucleic acid and proteins.In our approach we use a simple and cost effective wide-field imaging configuration with sequential illumination atmultiple wavelengths ranging from 220 nm to 450 nm. Spectral signatures are analyzed in conjunction with themorphology using a geometrical representation of principal component analysis and principles of mathematicalmorphology. Our results reveal distinct morphological and molecular alterations in the tissue as cancer becomes moreaggressive. In this presentation we will detail the design of the multispectral, deep UV microscope; describe ourquantitative image analysis; and show preliminary results.