Histone Deacetylase Inhibitor Corrects Historte H3K9 Modification in Round Spermatid DNA at the 2-Cell Stage and Increases ; the Development of ROSI Embryos .

摘要

Although round spermatic injection (ROSI) can be used as an assisted reproductive technology (ART) for treatment of male infertility, the successful rate of embryos and full-term development after ROSI is significantly lower than that for intracytoplasmic sperm injection (ICSI). In this study, we succeed in improving the potential of ROSI oocytes developed to the blastocyst stage and the quality of embryos by treatment of ROSI oocytes with histone deacetylase inhibitor (HDAC) scriptaid for 6 h after injection. ROSI oocytes treated with scriptaid prolong duration of histone H3K9 demethylation in round spermatid origin DNA until the 2-cell stage embryos. In contrast, high rate of ROSI embryos with histone methylated at H3K9 in round spermatid origin DNA is observed at the 2-cell stage embryos in group of ROSI oocytes without scriptaid treatment. Interestingly, our study also found that ROSI oocytes treated with scriptaid for 6 h after injection enhanced newly synthesized mRNA levels at the 2-cells stage embryos. In conclusion, this study showed that treatment of ROSI oocytes for 6 h after injection improved histone H3K9 modifications, transcriptional activity at the 2-cell stage embryos, and finally could increase the success rate of embryo development to the morula and blastocyst stage and the quality of ROSI blastocyst.