Recent studies suggest that cancer cell response to cisplatin can not be fully described in terms of only interaction of thedrug with DNA, but can include effects associated with other cellular targets. The study of effects of chemotherapeuticdrugs on the viscosity of plasma membrane is important for better understanding the mechanisms of the drug action andevaluating the effectiveness of therapy. The aim of this work was to analyze microviscosity of plasma membrane ofcancer cells during chemotherapy with cisplatin. For imaging viscosity at the microscopic level fluorescent molecularrotor BODIPY2 and fluorescence lifetime imaging microscopy (FLIM) were used. We detected a significant increase inmembrane viscosity in viable human cervical cancer cells HeLa, both in cell monolayer and tumor spheroids aftercisplatin treatment. Measuring viscosity in cisplatin-resistant cell line showed that viscosity increases when cellsacquire chemoresistance. These results suggest that microviscosity of membrane plays a role in the cytotoxicity ofcisplatin and its mapping may provide a powerful tool for investigation of tumor responses to chemotherapy andmechanisms of drug resistance.
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