ENGINEERING OF CARBOHYDRATE OXIDOREDUCTASES FOR SENSORS AND BIO-FUELCELLS

摘要

Pyranose dehydrogenase (PDH) and pyranose 2-oxidase (PO_x) are flavoproteins that catalyze the oxidation of free, non-phosphorylated sugars to the corresponding ketosugars. Pyranose dehydrogenase has a broad substrate specificity for monosaccharides (and few disaccharides), but is limited to a narrow range of electron acceptors and reacts extremely slowly with dioxygen, whereas pyranose 2-oxidase shows pronounced specificity for glucose and displays high oxidase as well as dehydrogenase activity.