COMPUTATIONAL DESIGN OF CATALYTICALLY ACTIVE TIM BARREL XYLANASES

摘要

TIM barrel enzymes perform five out of six Enzyme Commission (EC) reaction classes, and are therefore one of the most promising class of enzymes for computational design. Up until now, chimeric TIM barrels were made out of half barrels and catalytic activity was installed using laborious rounds of random mutagenesis, selection and rational design, whereas a recently described de novo designed TIM barrel lacked the elaborate loop conformations necessary to install catalytic activity, and indeed did not show activity.