Comparing Model Simulation and Experimental Results to Study the Dependence on Shear Stress of NO, ATP and ADP Production from Endothelial Cells

摘要

The concentrations of nitric oxide (NO) and adenine nucleotides (ATP, ADP) produced by cultured endothelial cells (ECs) in a parallel-plate flow chamber apparatus in response to changes in shear stress were compared to predictions generated by mathematical simulation. The model for NO production in a parallel flow chamber was based on earlier work, previously published by our group (1). Two models for adenine nucleotide production were used in the current study: a model developed by John and Barakat (2) and a model developed by Qin et al (3). The latter model was developed by fitting experimental results obtained by Yamamoto et al (4). The models were used to investigate the effect of changes in wall shear stress on production and transport of the three chemical species. In addition, experimental results using apyrase, which catalyses the hydrolysis of ATP to ADP and then to AMP, were compared to predictions of the simulations.