Effect of Isoelectric Point on Enzyme Immobilization Property of Magnetic Apatite Microcapsules Encapsulating Maghemite

摘要

It is well-known that apatite has high affinity to many kinds of biomolecules.In this study,the authors aimed to develop the apatite microcapsules with both enzyme immobilization property by the bioaffinity of apatite and magnetism of core materials.The authors encapsulated maghemite particles,possesing ferrimagnetism,with apatite by attaching Apatite Nuclei on the surfaces of maghemite particles and subsequently immersing them in SBF.To evaluate performance of the microcapsules as enzyme immobilization carriers,the authors investigated enzyme immobilization property of the microcapsules fabricated by biomimetic method using Apatite Nuclei and SBF from the viewpoint of the difference of isoelectric point of the three-types of enzymes.