SYNTHESIS OF TREHALOSE 6-PHOSPHATE AND ITS DERIVATIVE USING TREHALOSE 6-PHOSPHATE PHOSPHORYLASE

摘要

Trehalose 6-phosphate (Tre6P: a-D-Glcp-(1<->1)-o:-D-Glcp6P), the intermediate of trehalose biosynthesis, has profound regulatory functions on plant metabolism, growth, and development. However, availability of Tre6P is limited, because no efficient production system of Tre6P has been established thus far. Tre6P synthesis through fermentation by yeast was published, but the yield of Tre6P was low[1]. Therefore, in this study, we developed the efficient enzymatic synthesis of Tre6P using trehalose 6-phosphate phosphorylase (EC 2.4.1.216) from Lactococcus lactis ssp. lactis (LITrePP). In L lactis, LITrePP catalyzes the phosphorolysis of Tre6P, which is synthesized when trehalose is transported into cells by phosphotransferase system, to generate ss-glucose 1-phosphate (ss-Glc1P) and glucose 6-phosphate (Glc6P) [2]. Through a reverse reaction of phosphorolysis (synthesis), Tre6P and its derivative were synthesized.