Structure-based engineering of glycoenzymes for the development of chemo-enzymatic pathways to access bacterial carbohydrates

摘要

Combined with chemical synthesis, the use of biocatalysts holds great potential to open the way to molecular diversity. Nonetheless, the lack of appropriate enzymatic tools with requisite properties has prevented extensive exploration of chemo-enzymatic routes to complex carbohydrates. To circumvent this limitation, protein engineering has proven to be very efficient to tailor enzymes with novel substrate specificities. However, the outcome of protein engineering strategies strongly depends on our understanding of enzyme catalysis and our comprehension of the inter-relationships between protein structure, activity and dynamics.