The possible use of membrane filtration in the recovery process of plasmid DNA (pDNA) from fermentation broths, for applications in gene dierapy and DNA vaccine applications, has been studied in recent years. Application has been made to achieve efficient clarification from suspended solids after cell lysis and also to remove hydrophobic soluble contaminants such as RNA [1-5]. In our recent research, microfiltration and ultrafiltration have been performed in tandem to pre-purify and concentrate the plasmid before chromatography [6].
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