Research and Development of a Ganoderma lucidum Polysaccharide Injection Formulation

摘要

The aim of this study was to investigate the effect of injecting polysaccharides from Ganoderma lucidum into hosts for clinical practice. The polysaccharide aqueous extracts of Ganoderma lucidum' s fruit body was purified by Deproteinization of Sevage method, anion-exchange chromatography elution, dialysis, ethanol precipitation, active carbon filter, and millipore membrane filter technique. The injection was prepared from purified Ganoderma lucidum polysaccharide (GLPS). Polysaccharide indexes, protein, tannin, heavy metal, arsenic salt, oxalate, potassium ion, resin, pH, ignition residue, evaluation criterion of allergic reaction, total solids content of the GLPS injection were all tested with the reference method in Chinese Pharmacopoeia (2010ed). The results showed that the key component of injection was polysaccharide, ignition residue and total solids content were 1.4% and 2. 4%, respectively, the other indices were all within expected safety ranges. Notably, the injection improved the antifatigue capacity of mice with no effect in weight.