Disclosed by the present invention is a separation and purification method for polysaccharide in ganoderma lucidum spores, the method comprising: carrying out ultrasonic extraction on degreased ganoderma lucidum spore powder; and after frame filtration, carrying out precipitation and filtration on the obtained filtrate by means of ethanol having a mass concentration of 75% and ethanol having mass concentration of 85%. The obtained polysaccharide has an obvious effect for inhibiting the transplanted tumors of animals.
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