THE EFFECT OF LIPID BINDING PROTEIN AND ASPIRIN ON INFLAMMATION INDUCED BY LIPOPOLYSACCHARIDE

摘要

One of the hallmarks of inflammation is a pathological coagulation system also known as hypercoagulation. A highly potent inflammogen is known to be involved called lipopolysaccharide (LPS). LPS is located on the outer membrane of gram negative bacteria. We show that the addition of a tiny concentration (0.2 ng.L~(-1)) of bacterial LPS from E. coli (E. coli O111:B4 (Sigma, L2630)) to platelet-poor plasma (PPP) from healthy donors leads to marked changes in the nature of the fibrin network formed as seen from scanning electrone microsopy (SEM) and confocal microscopy. We also show that molecules such as LPS binding protein (LBP) and aspirin can reduce the effect of LPS. Research has shown that aspirin plays a role in coagulation as an antiplatelets agents. Whereas LBP has been reported to bind to LPS inhibiting the binding of LPS to plasma proteins.