SINGLE-CELL REAL-TIME PCR: DIRECT PROCESS FROM CELLS TO DATA

摘要

This paper presents an easily produced, cost-efficient chip-level device which has the ability to perform real-time PCR with single copy sensitivity. In order to integrate the high sensitivity and accuracy of real-time PCR and advantages of a miniaturized device, a simple chip-level device is built. We used this device to perform real-time PCR with single copy sensitivity. The minimum off-chip work will decrease the handling and human error during the whole process which will help to increase the successful rate and sensitivity of the chip.