OPTIMIZATION OF PSEUDOMONAS STUTZERI FOR PYRENE DEGRADATION UNDER DIFFERENT NUTRITIONAL AND ENVIRONMENTAL CONDITIONS

摘要

Pyrene which is a tetracyclic polyaromatic hydrocarbon with symmetrical structure has been classified as one of the priority pollutant by United State Environmental Protection Agency (USEPA). This compound is great concern because of its persistent toxicity and wide distribution in the environment. In order to eliminate pyrene, the bacterium capable of degrading pyrene was isolated from diesel contaminated soil. It was identified by biochemical characteristics, 16SrRNA gene sequence analysis and it showed 100% sequence similarity with Pseudomonas stutzeri. For efficient degradation of pyrene, various parameters such as temperature, pH and co-substrates (carbon and nitrogen) were optimized.