(00665)Bacterial Inactivation Study of Damages in Subcellular Level during Disinfection of Aqueous Samples

摘要

The aims of the study were i)to investigate ozonation and UVA/TiO2 photocatalysis as water disinfection techniches & ii)to study damages in subcellular level, in terms of disinfection effects on cellular components (lipids, membrane and proteins). Disinfection experiments were conducted with the bacterial strains Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus. Ozonation proved to be more effective for the disinfection of aqueous samples compared with UV-A photocatalysis, as for the Gram (-)bacteria a complete reduction was achieved within 15 min, whereas for the Gram (+)bacteria the same reduction was obtained within 30 min. Regarding the subcellular level the lipid peroxidation progressed at an exponential rate in the course of treatment. The ONPG hydrolysis assay showed negligible alterations in teh course of treatment, indicating that the cell membrane may act as an effective barrier between the cytoplasm and the outer solution in each case.