CTL Induction Using Cultured Dendritic Cells and Ibmor-RNA in Gastric Cancer

摘要

CTL induction using cultured dendritic cells (DCs) and tumor-RNA derived from gastric cancer (GC) was studied. DCs were generated from an adherent fraction of peripheral blood mononuclear cells (PBMCs) in the presence of GM-CSF and IL-4. mRNA was purified from a GC cell line or caner cells from GC patients, and subjected to in vitro TV-amplification. DCs were electroporated with amplified RNA and used for stimulating PBMCs to generate tumor RNA-introduced DC-activated killer (TRiDAK) cells. Tumor RNA-introduced DCs (RNA/DCs), but not normal cell-derived RNA/DCs, could stimulate lymphocytes. TRiDAK cells showed IFN-gamma spots and killing activity against tumor cells, but not against normal cells. TRiDAK activity was observed in an RNA-specific manner. TRiDAK response against tumor cells was abrogated with anti-class I or -class II antibodies, or with anti-TCR antibody. These results suggest that TRiDAK cell generation is feasible and active in patients with GC.