Synthetic Low Density Lipoprotein a Novel Biomimetic Lipid Supplement for Serum Free Tissue Culture

摘要

The provision of lipid components in aqueous based tissue culture media is difficult due to their limited solubility. Serum supplementation circumvents this but introduces contamination and consistency problems and non-serum lipid supplements are poor substitutes. To overcome these issues we have developed a new biomimetic lipid supplement, synthetic low density lipoprotein (sLDL), which is prepared by a cGMP compliant microfluidisation process using animal free components. sLDL is physically and chemically stable, filterable and can be prepared to contain a range of triglyceride and cholesterol ester components. sLDL cellular uptake is concentration, time and temperature dependent and undergoes competition with native LDL indicating a receptor dependent uptake via the LDL receptor. The cellular uptake permits sLDL to act as a lipid supplement and support the proliferation of cells in serum free media systems. sLDL therefore represents a novel biomimetic lipid supplement for serum free tissue culture, the exploration of cellular dietary lipid preferences and cellular delivery.