Improved enrichment of iodoTMT S-nitrosylated peptides using an anti-TMT antibody resin and competitive TMT Elution Buffer

摘要

iodoTMT Reagents enable multiplexed quantitation of cysteinecontaining peptides and cysteine PTMs Anti-TMT antibody has high affinity and specificity for the TMT mass tag reporter region and can enrich TMT-labeled proteins/peptides Competitive analogs were assessed to develop a new TMT Elution Buffer which increases TMT-labeled peptide enrichment specificity iodoTMT Reagents can be used in a modified switch assay for LC-MS identification and quantitation of S-nitrosylation site modifications