antibodies that immunospecifically bind to modified human igf-1 peptides, hybridoma, isolated polypeptide, nucleic acid encoding an antibody, vector comprising a nucleic acid, host cell comprising a vector, improved enzyme linked immunosorbent assay (elisa), method of maintaining optimal dose of modified igf-1 / e, improved affinity chromatographic method and use of modified igf-1 / e.

摘要

High-specificity antibodies can distinguish between modified (e.g, hIGF-1/Ea 3mut) and endogenous wild-type human IGF-1 proteins. These antibodies have little or no cross-reactivity with hIGF-1 or hIGF-2. They also have little or no cross-reactivity with rodent IGF-1 or IGF-2. The antibodies can be used in pharmacokinetic (PK)/pharamcodynamic (PD) assessments of IGF-1/E peptides that have been administered to humans or animals. A sandwich ELISA assay, using the antibody of the invention as a capture antibody, can quantify the mutant IGF-1/E proteins in samples.